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KMID : 0525619980030030222
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1998 Volume.3 No. 3 p.222 ~ p.227
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Production of Chitin and Chitosan Katabolism - Purification and Characteristics of Chitinase -
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차월석/Cha WS
차진명/Cha JM
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Abstract
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A chitinase-producing bacterium was isolated from seashore mud around Beobseongpo in Chunnam province by selective enrichment culture. The chitinase was purified by ammonium sulfate precipitation, affinity adsorption, Hydroxylapatite and Sephadex G-200 column chromatography. The chitinase extracted from Serratia marcescens KY was purified 42.2 folds with the overall yield of 7.1%. The purified chitinase showed a single band on sodium dodecy1 sulfate polyacrylamide gel electrophoresis and the molecular size was 59,000. The optimum pH and temperature of the purified chitinase were 7.0 and 50℃ and the purified enzyme was stable around pH 7.0 up to 50℃, respectively. The enzyme were activated by Cu2+, Ca2+and Mg2+and inhibited by Hg2+ and I2, respectively. In addition, cystein was increased for the chitinase activity and EDTA, MIA, PCMB and SDS inhibited for it.
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KEYWORD
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